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Advances in the study of directed evolution for cellulases

Hailong LIN, Weiguang LI, Changhong GUO, Sihang QU, Nanqi REN

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 4,   Pages 519-525 doi: 10.1007/s11783-011-0326-2

Abstract: Application of directed evolution technology may be one of the most effective ways for improving thehydrolysis mechanism by cellulase, advances in cellulases (endoglucanase and -glucosidase) improvement by directedcharacteristics (for instance, thermal stability, pH adaptability and enzyme activity), limitations of directedevolution for cellulases, and the outlook for directed evolution for cellulase.

Keywords： biohydrogen     bioethanol     cellulase     cellulose     directed evolution    

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Unnormalized and normalized forms of gefura measures in directed and undirected networks

Raf GUNS,Ronald ROUSSEAU

Frontiers of Information Technology & Electronic Engineering 2015, Volume 16, Issue 4,   Pages 311-320 doi: 10.1631/FITEE.1400425

Abstract: latter normalizes at the level of nodes. (2) Treating undirected networks as equivalent to symmetric directednetworks, we expand the definition of gefura measures to the directed case. (3) It is shown how Brandes

Keywords： Networks subdivided in groups     Partitions     Gefura measures     Q-measures     Brokerage role     Directed and    

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Controllability analysis of second-ordermulti-agent systemswith directed andweighted interconnection

Di GUO,Rong-hao ZHENG,Zhi-yun LIN,Gang-feng YAN

Frontiers of Information Technology & Electronic Engineering 2015, Volume 16, Issue 10,   Pages 838-847 doi: 10.1631/FITEE.1500069

Abstract: Each agent is assumed to be governed by a second-order consensus control law corresponding to a directedThe first is concerned with directed trees, which represent the class of topology with minimum informationThe second is concerned with a more general graph that can be reduced to a directed tree by contracting

Keywords： Multi-agent systems     Controllability     Directed tree    

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Ionic strength directed self-assembled polyelectrolyte single-bilayer membrane for low-pressure nanofiltration

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 5,   Pages 699-708 doi: 10.1007/s11705-021-2093-3

Abstract: In this work, an ionic strength directed self-assembly procedure is described for the preparation of

Keywords： layer-by-layer self-assembly     single bilayer     nanofiltration membrane     desalination    

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Liver-directed treatment is associated with improved survival and increased response to immune checkpoint

Frontiers of Medicine   Pages 878-888 doi: 10.1007/s11684-023-0993-y

Abstract: Due to low response rates to systemic therapies, liver-directed therapies (LDT) are commonly used for

Keywords： uveal melanoma     liver-directed therapy     immune checkpoint blockade     SIRT     anti-PD-1     anti-CTLA-4    

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Clinical outcomes of ATP-tumor chemosensitivity assay directed chemotherapy in hepatocellular carcinoma

RAO Rongsheng, CHEN Wenxue, ZHOU Xinwen, ZHOU Zheng, LI Xiaojun, ZENG Zhiping

Frontiers of Medicine 2007, Volume 1, Issue 2,   Pages 157-160 doi: 10.1007/s11684-007-0029-z

Abstract: This study aims to investigate the clinical response of ATP tumor chemosensitivity assay (ATP-TCA) directedIn short, ATP-TCA directed chemotherapy shows better results for terminal stages of PLC in that you can
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Site-directed mutagenesis of long QT syndrome KCNQ1 gene

LI Wei, WANG Bin, XU Qiumei, KE Qinmei, YANG Junguo, DU Rong, TIAN Li, WANG Qing

Frontiers of Medicine 2008, Volume 2, Issue 1,   Pages 100-104 doi: 10.1007/s11684-008-0018-x

Abstract: To construct a polymerase chain reaction (PCR) site-directed mutagenesis of the long QT syndrome KCNQ1

Keywords： restriction     digestion     syndrome     sequence     site-directed mutagenesis    

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oocyst directed assembly of gold nanoparticles and graphene oxide

Sona Jain, Zhicheng Huang, Brent R. Dixon, Syed Sattar, Juewen Liu

Frontiers of Chemical Science and Engineering 2019, Volume 13, Issue 3,   Pages 608-615 doi: 10.1007/s11705-019-1813-4

Abstract: Understanding the interactions between inorganic nanomaterials and biological species is an important topic for surface and environmental chemistry. In this work, we systematically studied the oocysts of as a model protozoan parasite and its interaction with gold nanoparticles (AuNPs) and graphene oxide (GO). The as-prepared citrate-capped AuNPs adsorb strongly on the oocysts leading to a vivid color change. The adsorption of the AuNPs was confirmed by transmission electron microscopy. Heat treatment fully inhibited the color change, indicating a large change of surface chemistry of the oocysts that can be probed by the AuNPs. Adding proteases such as trypsin and proteinase K partially inhibited the color change. DNA-capped AuNPs, on the other hand, could not be adsorbed by the oocysts. GO was found to wrap around the oocysts forming a conformal shell reflecting the shape of the oocysts. Both citrate-capped AuNPs and GO compromised the membrane integrity of the oocysts as indicated by the propidium iodide staining experiment, and they may be potentially used for inactivating the oocysts. This is the first example of using nanomaterials to probe the surface of the oocysts, and it suggests the possibility of using such organisms to template the assembly of nanomaterials.

Keywords： nanomaterials     toxicology     water     biosensors    

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Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B

Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen

Frontiers of Medicine 2015, Volume 9, Issue 1,   Pages 90-99 doi: 10.1007/s11684-015-0390-2

Abstract: therapy provides a potential cure for hemophilia B, and significant progress has been achieved in liver-directed

Keywords： factor IX     hemophilia B     liver-specific regulatory elements     hydrodynamic gene transfer    

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Minimal residual disease-directed immunotherapy for high-risk myelodysplastic syndrome after allogeneic

Xiaodong Mo, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Wei Han, Fengrong Wang, Jingzhi Wang, Kaiyan Liu, Xiaojun Huang

Frontiers of Medicine 2019, Volume 13, Issue 3,   Pages 354-364 doi: 10.1007/s11684-018-0665-5

Abstract: The efficacy of minimal residual disease (MRD)-directed immunotherapy, including interferon- (IFN- )Thus, the MRD-directed immunotherapy was effective for patients with high-risk MDS who were MRD-positive

Keywords： donor leukocyte infusion     hematopoietic stem cell transplantation     interferon-   

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Development of a dual temperature control system for isoprene biosynthesis in

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 7,   Pages 1079-1089 doi: 10.1007/s11705-021-2088-0

Abstract: A cold-sensitive mutant, Gal4ep19, was created by directed evolution of Gal4p based on an internallyThis study reports the creation of the first cold-sensitive variants of Gal4p by directed evolution and

Keywords： transcriptional activator     directed evolution     dynamic control     heat-shock     isoprene    

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DNA alkylation promoted by an electron-rich quinone methide intermediate

Chengyun Huang,Steven E. Rokita

Frontiers of Chemical Science and Engineering 2016, Volume 10, Issue 2,   Pages 213-221 doi: 10.1007/s11705-015-1541-3

Abstract: Biological application of conjugates derived from oligonucleotides and quinone methides have previously been limited by the slow exchange of their covalent self-adducts and subsequent alkylation of target nucleic acids. To enhance the rates of these processes, a new quinone methide precursor with an electron donating substituent has been prepared. Additionally, this substituent has been placed to the nascent -methylene group of the quinone methide for maximum effect. A conjugate made from this precursor and a 5'-aminohexyloligonucleotide accelerates formation of its reversible self-adduct and alkylation of its complementary DNA as predicted from prior model studies.

Keywords： quione methide     DNA alkylation     reversible covalent reaction     bioconjugation     target-directed modification    

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Cofactor engineering in cyanobacteria to overcome imbalance between NADPH and NADH: A mini review

Jongmoon Park,Yunnam Choi

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 66-71 doi: 10.1007/s11705-016-1591-1

Abstract: Cyanobacteria can produce useful renewable fuels and high-value chemicals using sunlight and atmospheric carbon dioxide by photosynthesis. Genetic manipulation has increased the variety of chemicals that cyanobacteria can produce. However, their uniquely abundant NADPH-pool, in other words insufficient supply of NADH, tends to limit their production yields in case of utilizing NADH-dependent enzyme, which is quite common in heterotrophic microbes. To overcome this cofactor imbalance and enhance cyanobacterial fuel and chemical production, various approaches for cofactor engineering have been employed. In this review, we focus on three approaches: (1) utilization of NADPH-dependent enzymes, (2) increasing NADH production, and (3) changing cofactor specificity of NADH-dependent enzymes from NADH to NADPH.

Keywords： NADH-dependent enzyme     NADPH-dependent enzyme     transhydrogenase     site-directed mutagenesis     enzyme engineering    

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with acute leukemia/myelodysplastic syndrome with unsatisfactory response to minimal residual disease-directed

Xiaodong Mo, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Wei Han, Fengrong Wang, Jingzhi Wang, Kaiyan Liu, Xiaojun Huang

Frontiers of Medicine 2019, Volume 13, Issue 2,   Pages 238-249 doi: 10.1007/s11684-017-0599-3

Abstract: treatment was investigated in patients with unsatisfactory response to minimal residual disease (MRD)-directedsalvage IFN-α treatment may help improve the outcome of patients with unsatisfactory responses to MRD-directed

Keywords： interferon-α     hematopoietic stem cell transplantation     minimal residual disease     donor lymphocyte infusion    

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cooperative control of multipleUAVs with prescribed attitude synchronization tracking performance under directed Regular Papers

Zi-quan YU, Zhi-xiang LIU, You-min ZHANG, Yao-hong QU, Chun-yi SU

Frontiers of Information Technology & Electronic Engineering 2019, Volume 20, Issue 5,   Pages 685-700 doi: 10.1631/FITEE.1800569

Abstract: is developed for multiple unmanned aerial vehicles (UAVs) in the presence of actuator faults and a directed

Keywords： Decentralized control     Prescribed performance     Unmanned aerial vehicle     Neural network     Disturbance observer     Directed    

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Title Author Date Type Operation

Advances in the study of directed evolution for cellulases

Hailong LIN, Weiguang LI, Changhong GUO, Sihang QU, Nanqi REN

Journal Article

Unnormalized and normalized forms of gefura measures in directed and undirected networks

Raf GUNS,Ronald ROUSSEAU

Journal Article

Controllability analysis of second-ordermulti-agent systemswith directed andweighted interconnection

Di GUO,Rong-hao ZHENG,Zhi-yun LIN,Gang-feng YAN

Journal Article

Ionic strength directed self-assembled polyelectrolyte single-bilayer membrane for low-pressure nanofiltration

Journal Article

Liver-directed treatment is associated with improved survival and increased response to immune checkpoint

Journal Article

Clinical outcomes of ATP-tumor chemosensitivity assay directed chemotherapy in hepatocellular carcinoma

RAO Rongsheng, CHEN Wenxue, ZHOU Xinwen, ZHOU Zheng, LI Xiaojun, ZENG Zhiping

Journal Article

Site-directed mutagenesis of long QT syndrome KCNQ1 gene

LI Wei, WANG Bin, XU Qiumei, KE Qinmei, YANG Junguo, DU Rong, TIAN Li, WANG Qing

Journal Article

oocyst directed assembly of gold nanoparticles and graphene oxide

Sona Jain, Zhicheng Huang, Brent R. Dixon, Syed Sattar, Juewen Liu

Journal Article

Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B

Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen

Journal Article

Minimal residual disease-directed immunotherapy for high-risk myelodysplastic syndrome after allogeneic

Xiaodong Mo, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Wei Han, Fengrong Wang, Jingzhi Wang, Kaiyan Liu, Xiaojun Huang

Journal Article

Development of a dual temperature control system for isoprene biosynthesis in

Journal Article

DNA alkylation promoted by an electron-rich quinone methide intermediate

Chengyun Huang,Steven E. Rokita

Journal Article

Cofactor engineering in cyanobacteria to overcome imbalance between NADPH and NADH: A mini review

Jongmoon Park,Yunnam Choi

Journal Article

with acute leukemia/myelodysplastic syndrome with unsatisfactory response to minimal residual disease-directed

Xiaodong Mo, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Wei Han, Fengrong Wang, Jingzhi Wang, Kaiyan Liu, Xiaojun Huang

Journal Article

cooperative control of multipleUAVs with prescribed attitude synchronization tracking performance under directed

Zi-quan YU, Zhi-xiang LIU, You-min ZHANG, Yao-hong QU, Chun-yi SU

Journal Article